{"uid":"cap_xbNv9DaSsnp_gwBpLgyKr","slug":"bio-halowerk-com-allergen-epitope-similarity-analyzer-8ee11ef9","name":"bio.halowerk.com Allergen Epitope Similarity Analyzer","description":"Builds amino-acid k-mer sets, calculates Jaccard similarity, and finds the highest contiguous identity between each supplied epitope and any equal-length window of the query. It does not predict immune binding, allergenicity, cross-reactivity or clinical risk and cannot replace curated databases or laboratory testing.","url":"https://bio.halowerk.com/v1/allergen-epitope","method":"POST","headers":{},"bodySchema":{"type":"object","properties":{"kmer_size":{"type":"integer","maximum":12,"minimum":2},"known_epitopes":{"type":"array","items":{"type":"object","required":["id","sequence"],"properties":{"id":{"type":"string","maxLength":128,"minLength":1},"sequence":{"type":"string","pattern":"^[ACDEFGHIKLMNPQRSTVWY]+$","maxLength":500,"minLength":2}},"additionalProperties":false},"maxItems":200,"minItems":1},"query_sequence":{"type":"string","pattern":"^[ACDEFGHIKLMNPQRSTVWY]+$","maxLength":10000,"minLength":2}}},"responseSchema":null,"example":null,"exampleRequest":null,"tags":["x402"],"displayCostAmount":"0.004","displayCostAsset":"USDC","priceDynamic":false,"priceHint":null,"priceStatus":"priced","priceSource":"probe","requiresHandshake":false,"reviewCount":0,"rating":{"score":"0.00","successRate":"0.00","reviews":0,"stars":null,"state":"unrated"},"availabilityStatus":"unknown","priceObserved":null,"sessionDeposit":null,"pricing":{"kind":"static","summary":"$0.004/call","primary":{"kind":"static","protocol":"x402","network":"base","amountUsd":"0.004","per":"call","confidence":"exact"},"accepted":[{"kind":"static","protocol":"x402","network":"base","amountUsd":"0.004","per":"call","confidence":"exact"}]},"paymentMethods":[{"uid":"pm_mveErBWe7VCmmClCOpWAj","protocol":"x402","methodType":"crypto","chain":"base","mode":"charge","costAmount":"0.004","costPer":"request","priority":0,"asset":"0x833589fCD6eDb6E08f4c7C32D4f71b54bdA02913","unit":"request","depositMicros":null,"planRef":null}],"brandName":null,"brandSlug":null,"brandBaseUrl":null,"brandDocsUrl":null,"whatItDoes":"Builds amino-acid k-mer sets from a query protein sequence and known epitopes, computes Jaccard similarity, and finds the highest contiguous identity window between each epitope and the query sequence.","exampleAgentPrompt":"Compare this protein sequence MKTIIALSYIFCLVFA...RNDEQK against these 5 known allergen epitopes — use a k-mer size of 6 — and tell me the Jaccard similarity and highest contiguous identity for each epitope.","exampleUseCases":[{"title":"Novel food protein allergenicity screening","prompt":"I've got a candidate recombinant soy protein with sequence MKTLLLTLVVVTIVCLDLGAVGNGLCCY and I want to compare it against these 12 known soy allergen epitopes using k-mer size 8 — tell me the Jaccard similarity and best contiguous identity match for each one."},{"title":"Bioinformatics pipeline epitope overlap check","prompt":"Run a k-mer comparison with k=5 between my query protein ACDEFGHIKLMNPQRSTVWYACDEF and these three reference epitopes — IDs epi001, epi002, epi003 — and give me the Jaccard scores and highest identity windows."},{"title":"Research-grade sequence similarity for epitope mapping","prompt":"I'm studying cross-reactive proteins and need to know how similar the sequence RNDEQKLMVWPFGSTYA is to each of these 20 known epitopes — use k-mer size 4 and report back the Jaccard similarity and maximum contiguous match length for every epitope."}],"resultDescription":"Returns per-epitope results including Jaccard similarity score (based on k-mer set overlap) and the highest contiguous identity found between the epitope and any equal-length window in the query sequence. Does not include immune binding predictions, allergenicity risk scores, or clinical assessments.","failureModes":["Invalid amino acid characters in query_sequence or epitope sequences (must match ^[ACDEFGHIKLMNPQRSTVWY]+$)","kmer_size outside allowed range of 2–12 returns validation error","known_epitopes array empty or exceeds 200 items","query_sequence or epitope sequence shorter than 2 characters","Epitope sequence longer than 500 characters or query longer than 10000 characters","Missing required fields (query_sequence or known_epitopes)"],"whenToPreferThis":"Use this endpoint when you need a fast, computational sequence-similarity comparison between a query protein and a set of known epitopes using k-mer and contiguous identity methods. It is appropriate for bioinformatics screening workflows, research pipelines, or preliminary similarity assessments — not for clinical allergenicity prediction, immune binding affinity prediction, or as a replacement for curated allergen databases.","instructions":null,"reviewSummary":null,"reviewSummaryHighlights":null,"reviewSummaryConcerns":null,"reviewSummaryGeneratedAt":null,"activationCount":0,"lastUsedAt":null,"lastSuccessfullyRanAt":null,"lastHealthCheckAt":"2026-09-14T00:37:57.943Z","isFirstParty":false}