{"uid":"cap_qw7n43E2P4s_Mw-vA-iUv","slug":"magent-dli-whole-blood-volume-calculator-shi-2020-e4ee3302","name":"Magent DLI Whole-Blood Volume Calculator (Shi 2020)","description":"Shi 2020 donor whole-blood liters to process for a CD3+ DLI target: (recipient_weight_kg × cd3_target_per_kg × num_infusions) / (0.6 × donor_alc_per_ul × collection_efficiency × 1e6). Estimates CD3 as 60% of donor ALC. Caller assigns collection_efficiency in (0,1]; the paper used first-quartile 0.57. Deterministic published arithmetic with citation. Not an apheresis order; magent does not run a device or the paper's CD34 HPC algorithm.","url":"https://api.magentlab.com/api/calc/dli_volume","method":"GET","headers":{},"bodySchema":{"type":"object","$schema":"https://json-schema.org/draft/2020-12/schema","required":["input"],"properties":{"input":{"type":"object","required":["type","method"],"properties":{"type":{"type":"string","const":"http"},"method":{"enum":["GET","HEAD","DELETE"],"type":"string"},"headers":{"type":"object","additionalProperties":{"type":"string"}},"queryParams":{"type":"object","required":["recipient_weight_kg","cd3_target_per_kg","donor_alc_per_ul","collection_efficiency"],"properties":{"num_infusions":{"type":"integer","description":"Optional integer infusions (1-12, default 1) multiplying the per-kg CD3 target when the planned dose is the total across infusions. Shi 2020 collected one 2e8/kg DLI product."},"donor_alc_per_ul":{"type":"number","description":"Donor absolute lymphocyte count on the collection day, cells/µL (100-20000, magent clamp). Shi 2020 estimates CD3+ as 60% of this ALC rather than a measured CD3 count. magent does not assay ALC."},"cd3_target_per_kg":{"type":"number","description":"Planned CD3+ cells per recipient kilogram (1e6-1e9, magent clamp). Shi 2020's haploidentical protocol used 2e8/kg; a first DLI is often 1e7/kg. Not a CD34 HPC dose."},"recipient_weight_kg":{"type":"number","description":"Recipient weight in kilograms (1-500, magent clamp). Shi 2020 doses CD3 per recipient kg. magent does not weigh the recipient."},"collection_efficiency":{"type":"number","description":"Caller-assigned facility lymphocyte CE2 as a proportion in (0, 1]. Shi 2020 used a conservative first-quartile 0.57; magent does not default to 0.57 or invent a CE."}}}},"additionalProperties":false},"output":{"type":"object","required":["type"],"properties":{"type":{"type":"string"},"example":{"type":"object"}}}}},"responseSchema":null,"example":null,"exampleRequest":null,"tags":["x402"],"displayCostAmount":"0.005","displayCostAsset":"USDC","priceDynamic":false,"priceHint":null,"priceStatus":"priced","priceSource":"probe","requiresHandshake":false,"reviewCount":0,"rating":{"score":"0.00","successRate":"0.00","reviews":0,"stars":null,"state":"unrated"},"availabilityStatus":"unknown","priceObserved":null,"sessionDeposit":null,"pricing":{"kind":"static","summary":"$0.005/call","primary":{"kind":"static","protocol":"x402","network":"base","amountUsd":"0.005","per":"call","confidence":"exact"},"accepted":[{"kind":"static","protocol":"x402","network":"base","amountUsd":"0.005","per":"call","confidence":"exact"}]},"paymentMethods":[{"uid":"pm_OgrBSK3GteALZWXSu-kr3","protocol":"x402","methodType":"crypto","chain":"base","mode":"charge","costAmount":"0.005","costPer":"request","priority":0,"asset":"0x833589fCD6eDb6E08f4c7C32D4f71b54bdA02913","unit":"request","depositMicros":null,"planRef":null}],"brandName":null,"brandSlug":null,"brandBaseUrl":null,"brandDocsUrl":null,"whatItDoes":"Calculates the volume of donor whole blood to process by apheresis to achieve a target CD3+ cell dose for a donor lymphocyte infusion (DLI), using the Shi 2020 formula","exampleAgentPrompt":"Using the Shi 2020 DLI formula, calculate how many liters of whole blood to process for a recipient who weighs 70 kg, targeting a CD3 dose of 2e8 cells/kg for 1 infusion, given the donor's ALC is 1800 cells/µL and our facility's collection efficiency is 0.57.","exampleUseCases":[{"title":"Haploidentical DLI planning for transplant team","prompt":"Our 65 kg transplant patient needs a haploidentical DLI at 2e8 CD3+ cells/kg for a single infusion. The donor's ALC today is 2200 cells/µL and our apheresis lab runs at a collection efficiency of 0.57. Can you calculate the whole-blood volume to process using the Shi 2020 method?"},{"title":"Low-dose first DLI for relapse prevention","prompt":"We're planning a low-dose first DLI at 1e7 CD3+ cells/kg for a 55 kg recipient. The donor ALC is 1500 cells/µL and we'll use a CE of 0.65. How many liters of whole blood does the Shi 2020 formula say we need to run through apheresis?"},{"title":"Multi-infusion DLI collection planning","prompt":"We need to collect enough for 3 DLI infusions targeting 5e7 CD3+ cells/kg each for a 80 kg patient. Donor ALC is 1900 cells/µL and collection efficiency is 0.57. What total blood volume should we plan to process according to the Shi 2020 calculation?"}],"resultDescription":"Returns the estimated volume of whole blood (in liters) the apheresis machine must process to yield the desired CD3+ cell count, computed as (recipient_weight_kg × cd3_target_per_kg × num_infusions) / (0.6 × donor_alc_per_ul × collection_efficiency × 1e6). CD3+ count is estimated as 60% of the donor ALC per the Shi 2020 paper. Input values are clamped to clinically valid ranges.","failureModes":["Missing required parameters (recipient_weight_kg, cd3_target_per_kg, donor_alc_per_ul, collection_efficiency) returns an error","collection_efficiency of 0 causes division by zero — value must be in (0, 1]","Values outside clamped ranges (e.g. donor_alc_per_ul outside 100–20000) may be clamped or rejected","num_infusions outside 1–12 integer range may be rejected","Payment failure via x402 protocol returns 402 status"],"whenToPreferThis":"Use this endpoint when you need a fast, deterministic, citation-backed calculation of whole-blood apheresis volume for CD3+ DLI planning per the Shi 2020 haploidentical protocol. Prefer it over manual spreadsheets when integrating into clinical decision-support agents or transplant workflow automation. Note it does not compute CD34 HPC collection volumes (a different algorithm) and does not control or interface with any apheresis device.","instructions":null,"reviewSummary":null,"reviewSummaryHighlights":null,"reviewSummaryConcerns":null,"reviewSummaryGeneratedAt":null,"activationCount":0,"lastUsedAt":null,"lastSuccessfullyRanAt":null,"lastHealthCheckAt":"2026-09-15T13:02:20.272Z","isFirstParty":false}