{"uid":"cap_OO7XP01qULhL8qi7mm5OF","slug":"qpcr-run-quality-check-a054aa46","name":"qPCR Run Quality Check","description":"Check supplied Ct replicates, efficiency and control declarations against fixed research QC bands; report Ct SD and failed conditions. No diagnostic validation. New modular adapter: retained result recovery for 24 hours, minimal receipt metadata for 90 days. Existing live route unchanged.","url":"https://www.mahastrategies.com/api/v1/compute/biology/qpcr-run-quality?utm_source=zero.xyz","method":"POST","headers":{},"bodySchema":{"type":"object","properties":{"ct":{"type":"array","maxItems":128,"minItems":1},"dataClass":{"enum":["public","synthetic"],"type":"string"},"efficiency":{"type":"number","maximum":1000000000000,"minimum":-1000000000000},"noRTNegative":{"type":"boolean"},"meltSinglePeak":{"type":"boolean"},"referenceStable":{"type":"boolean"},"noTemplateNegative":{"type":"boolean"}}},"responseSchema":null,"example":null,"exampleRequest":null,"tags":["x402"],"displayCostAmount":"0.010042","displayCostAsset":"USDC","priceDynamic":false,"priceHint":null,"priceStatus":"priced","priceSource":"probe","requiresHandshake":false,"reviewCount":0,"rating":{"score":"0.00","successRate":"0.00","reviews":0,"stars":null,"state":"unrated"},"availabilityStatus":"unknown","priceObserved":null,"sessionDeposit":null,"pricing":{"kind":"static","summary":"$0.010042/call","primary":{"kind":"static","protocol":"x402","network":"base","amountUsd":"0.010042","per":"call","confidence":"exact"},"accepted":[{"kind":"static","protocol":"x402","network":"base","amountUsd":"0.010042","per":"call","confidence":"exact"}]},"paymentMethods":[{"uid":"pm_aTOOkQGS0MHrgk7oSowp9","protocol":"x402","methodType":"crypto","chain":"base","mode":"charge","costAmount":"0.010042","costPer":"request","priority":0,"asset":"0x833589fCD6eDb6E08f4c7C32D4f71b54bdA02913","unit":"request","depositMicros":null,"planRef":null}],"brandName":null,"brandSlug":null,"brandBaseUrl":null,"brandDocsUrl":null,"whatItDoes":"Validates qPCR run data (Ct replicates, amplification efficiency, and control flags) against fixed research QC thresholds and reports Ct standard deviation plus any failed conditions.","exampleAgentPrompt":"Can you run a QC check on my qPCR data? The Ct values across my replicates are [22.1, 22.4, 22.3, 22.6], amplification efficiency is 95.5%, the no-template negative control passed, the no-RT negative control passed, I have a single melt peak, and my reference gene is stable — data class is synthetic.","exampleUseCases":[{"title":"Gene expression replicate QC","prompt":"I have qPCR Ct replicates of [28.1, 28.4, 28.0, 28.7] from a gene expression experiment with 97% amplification efficiency, a passing no-template control, a single melt peak, and a stable reference gene — can you check whether these replicates meet research QC standards and tell me what failed?"},{"title":"Screen for failed NTC control","prompt":"Check my qPCR run quality: Ct values are [30.2, 30.5, 30.1], efficiency is 85%, but I did not observe a clean no-template negative control — noTemplateNegative is false. The no-RT control passed and the melt curve shows a single peak. Flag any QC failures for me."},{"title":"Low-efficiency run assessment","prompt":"I want to know if my qPCR run passes QC — the Ct replicates are [19.8, 20.1, 19.9], but the amplification efficiency is only 60%, both negative controls passed, reference gene is stable, and I see a single melt peak. Data class is public."}],"resultDescription":"Returns the computed Ct standard deviation across the supplied replicates, a list of specific QC conditions that failed (e.g. high Ct SD, out-of-range efficiency, failed controls), and an overall QC pass/fail indication. Results are retained for 24 hours; minimal receipt metadata is stored for 90 days.","failureModes":["Fewer than 1 or more than 128 Ct values supplied — validation error","Efficiency value outside the acceptable numeric range — rejected input","Missing required fields such as dataClass — schema validation failure","Ct values with extreme outliers may produce high SD flags that are not indicative of a true run failure","Ambiguous control states (e.g. null boolean flags) may be treated as absent rather than passed"],"whenToPreferThis":"Use this endpoint when you need automated, rule-based QC assessment of a qPCR run against fixed research thresholds — particularly when you want a structured report of Ct SD and specific failed conditions without needing a clinical diagnostic validation framework. Prefer this over manual spreadsheet checks or general statistics endpoints when the input is specifically Ct replicate arrays combined with efficiency and control metadata.","instructions":null,"reviewSummary":null,"reviewSummaryHighlights":null,"reviewSummaryConcerns":null,"reviewSummaryGeneratedAt":null,"activationCount":0,"lastUsedAt":null,"lastSuccessfullyRanAt":null,"lastHealthCheckAt":"2026-10-28T06:47:08.181Z","isFirstParty":false,"canonicalSlug":"qpcr-run-quality-check-a054aa46"}